Development of 2,3-Butanediol Production Process from Klebsiella aerogenes ATCC 29007 Using Extracted Sugars of Chlorella pyrenoidosa and Biodiesel-Derived Crude Glycerol

Expectation for renewable energy is increasing due to environmental pollution such as fossil fuel depletion, CO2 emission, and harmful gases. Therefore, in this study, extracted sugars of microalgae, which cause algal blooms crude glycerol, a biodiesel industry byproduct, were used simultaneously produce 2,3-BDO. The 2,3-BDO production using only was about 4.8 g/L at 18 h, the both sugar glycerol 7 h. It confirmed that main culture with increased 1.5-fold compared case sugars. In addition, four components medium (ammonium sulfate, casein hydrolysate, yeast extract, glycerol) statistically optimized concentrations 12, 16, 13 g/L, respectively. final 11g/L, 1.6-fold higher than before optimization process. As result, it possible through simultaneous use can greatly contribute development zero-waste processes.